Pyridoxal 5′-phosphate hydrate is widely used as a coenzyme in various metabolic processes, particularly in amino acid metabolism. It plays a crucial role in transamination, decarboxylation, and deamination reactions, making it essential for the synthesis and breakdown of amino acids. This compound is also involved in the production of neurotransmitters such as serotonin, dopamine, and gamma-aminobutyric acid (GABA), which are vital for proper brain function. Additionally, it supports the conversion of homocysteine to cysteine, helping to maintain cardiovascular health. In the pharmaceutical industry, it is utilized in supplements to treat vitamin B6 deficiencies and related conditions. Furthermore, it finds applications in research laboratories as a reagent for studying enzymatic reactions and metabolic pathways.