TNBT Diformazan is primarily utilized in biological staining and histochemical applications. It serves as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISAs) and immunohistochemistry, where it helps visualize and detect specific proteins or enzymes. Its ability to produce a distinct color change upon reaction makes it valuable for identifying cellular components and studying enzyme activity in tissue samples. Additionally, it is employed in research to assess oxidative stress and antioxidant activity in biological systems, providing insights into cellular health and function.