This chemical is widely used as a substrate in enzymatic assays, particularly for detecting the activity of sulfatase enzymes. When sulfatase enzymes act on it, the compound is cleaved to produce an insoluble blue precipitate, making it valuable for histochemical staining and visualizing enzyme activity in tissues or cells. It is also employed in molecular biology and biochemistry research to study enzyme kinetics and localization. Additionally, it serves as a critical component in diagnostic kits for identifying sulfatase-related disorders. Its high sensitivity and specificity make it a preferred choice for applications requiring precise detection of enzymatic activity.