Used extensively as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISAs) for detecting peroxidase activity. Its application is crucial in diagnostic kits for identifying various diseases, including infectious diseases and cancer biomarkers. The compound changes color upon oxidation, providing a visual or spectrophotometric signal that correlates with the presence and concentration of the target analyte. Additionally, it is employed in biochemical research to study enzyme kinetics and mechanisms involving peroxidase enzymes. Its sensitivity and stability make it a preferred choice in both clinical and laboratory settings for accurate and reliable results.