Used as a chromogenic substrate in biochemical assays, particularly in enzyme-linked immunosorbent assays (ELISA) and phosphatase detection. It is especially effective for detecting alkaline phosphatase activity, where the enzyme cleaves the phosphate group, releasing a yellow-colored product that can be measured spectrophotometrically. This color change allows for quantitative analysis of enzyme activity, making it valuable in diagnostic testing, research, and immunoassays. The compound provides high sensitivity and stability, suitable for use in various clinical and laboratory applications.