Used as a chromogenic substrate in enzymatic assays to detect and quantify β-glucosidase activity. Upon hydrolysis by β-glucosidase, it releases p-nitrophenol, which produces a yellow color detectable at 400–410 nm, enabling real-time monitoring of enzyme kinetics. Commonly employed in biochemical research, microbial studies, and industrial enzyme testing where color development indicates enzyme presence and activity. Its acetylated sugar group enhances substrate stability and membrane permeability in certain biological systems.