1-Naphthyl phosphate potassium salt is primarily used as a chromogenic substrate for alkaline phosphatase (ALP) in enzyme assays such as ELISA, histochemical staining, and biochemical research. It is hydrolyzed by ALP to release 1-naphthol, which can be detected colorimetrically or fluorometrically for quantifying enzyme activity. This compound is valuable for studying phosphatase-mediated dephosphorylation processes, developing diagnostic kits, and monitoring phosphate group removal in biomolecules. Its good solubility and stability in aqueous solutions make it suitable for use in buffer systems during enzymatic studies. Additionally, it serves as an intermediate in the synthesis of specific organophosphate compounds for research applications.