Used as a reagent in analytical chemistry for the detection and estimation of oxidative enzymes such as peroxidases and oxidases. It serves as a chromogenic substrate that produces a visible color change upon oxidation, enabling easy monitoring of enzyme activity in clinical and research laboratories. Commonly applied in diagnostic tests, including those for measuring glucose, cholesterol, and other metabolites in blood and urine. Also utilized in histology and microbiology for staining procedures to identify specific enzyme-producing cells or microorganisms. Its high sensitivity and clear endpoint make it valuable in colorimetric assays.