Used as a key intermediate in the synthesis of organophosphorus compounds, particularly in the development of fluorescent probes and sensors for detecting enzymes like phosphatases in biological systems. Its nitro and dimethylamino groups enable strong chromogenic responses, making it useful in colorimetric assays. Also employed in pesticide research due to its reactivity with serine hydrolases, aiding in the design of enzyme inhibitors. Shows potential in agrochemical formulations as a prodrug moiety that releases active ingredients upon enzymatic activation.