Used in immunohistochemistry (IHC) and in situ hybridization (ISH) as a chromogen to visualize the presence of specific antigens or nucleic acid sequences in tissue sections. The solution produces a brown precipitate at the site of target detection, offering high contrast against counterstains, which makes it ideal for brightfield microscopy. Its ready-to-use, two-component format simplifies preparation and ensures consistent staining performance. DAB is compatible with both manual and automated staining systems, widely preferred in diagnostic pathology and research for reliable and sensitive detection.