This chemical is primarily used as a reagent in enzymatic assays, particularly in the detection of hydrogen peroxide. It serves as a chromogenic substrate in biochemical research, where it reacts with peroxidase enzymes to produce a measurable color change, enabling the quantification of enzymatic activity. This makes it valuable in diagnostic applications, such as glucose testing in clinical laboratories, where it helps determine glucose levels by measuring the hydrogen peroxide generated during glucose oxidation. Additionally, it is employed in various research settings to study oxidative stress and antioxidant activity in biological systems. Its stability and sensitivity make it a reliable choice for precise analytical measurements.