MTT is widely used in cell biology and biomedical research as a key reagent for assessing cell viability and proliferation. It is particularly valuable in the MTT assay, where it serves as a substrate to measure mitochondrial activity in living cells. When added to cell cultures, MTT is reduced by metabolically active cells into a purple formazan product, which can be quantified spectrophotometrically. This assay is commonly employed in drug screening, cytotoxicity studies, and cancer research to evaluate the effects of various compounds on cell growth and survival. Its simplicity, reliability, and sensitivity make it a staple in laboratories studying cellular responses to treatments or environmental conditions.