NBTGR is primarily utilized in the field of biochemistry and molecular biology as a reagent for detecting and quantifying the presence of specific enzymes, particularly those involved in redox reactions. It is often employed in assays to measure the activity of enzymes like dehydrogenases, where it acts as an electron acceptor, leading to the formation of a colored product that can be easily quantified using spectrophotometry. This makes it a valuable tool in research focused on cellular metabolism, oxidative stress, and enzymatic pathways. Additionally, NBTGR is used in histochemistry for staining tissues to visualize enzyme activity, aiding in the study of cellular and tissue functions under various physiological and pathological conditions. Its application extends to diagnostic testing, where it helps in identifying enzyme deficiencies or abnormalities in clinical samples.