Column-layer chromatographic silica gel is widely used in the separation and purification of complex mixtures in both analytical and preparative chemistry. It serves as a stationary phase in column chromatography, allowing for the efficient separation of compounds based on their polarity, size, or affinity. This material is particularly valuable in the pharmaceutical industry for isolating and purifying active pharmaceutical ingredients (APIs) from crude extracts. In biochemistry, it is employed for the purification of proteins, peptides, and nucleic acids. Additionally, it is utilized in the food industry to separate and analyze additives, contaminants, or natural compounds. Its high surface area and tunable pore size make it versatile for applications in environmental testing, such as the analysis of pollutants in water or soil samples. The silica gel's ability to provide high-resolution separation makes it a critical tool in research and quality control laboratories.