Column-layer chromatographic silica gel is widely used in the separation and purification of complex mixtures in both analytical and preparative chemistry. Its high surface area and porosity make it ideal for adsorbing and separating compounds based on their polarity and molecular size. In pharmaceuticals, it is employed to isolate and purify active ingredients from raw extracts, ensuring high purity and quality. In biochemistry, it aids in the separation of proteins, peptides, and nucleic acids, facilitating research and diagnostic applications. Additionally, it is utilized in environmental analysis to separate and identify pollutants in water, soil, and air samples. Its versatility and efficiency make it a crucial tool in various scientific and industrial processes.