Monodisperse amino silica microspheres are widely used in chromatography for the separation and purification of biomolecules such as proteins, peptides, and nucleic acids. Their uniform size and surface functionalization with amino groups enhance binding efficiency and resolution. In drug delivery systems, these microspheres serve as carriers for controlled release of therapeutic agents due to their biocompatibility and tunable surface properties. They are also employed in catalysis as supports for immobilizing enzymes or metal catalysts, improving stability and reusability. Additionally, their application in diagnostics includes their use as substrates for biosensors, where the amino groups facilitate the attachment of biomolecules for sensitive detection. In environmental science, they are utilized for the adsorption and removal of pollutants from water, leveraging their high surface area and functional groups for effective capture.