Valinomycin is widely used in biochemical research as a potassium ionophore. It selectively transports potassium ions across lipid membranes, making it a valuable tool in studying ion transport mechanisms and membrane potential. In cellular biology, it is employed to manipulate intracellular potassium levels, helping researchers understand the role of potassium in cell signaling, apoptosis, and mitochondrial function. Additionally, valinomycin is used in ion-selective electrodes to detect potassium ions in various samples, including biological fluids and environmental samples. Its ability to disrupt mitochondrial membrane potential also makes it useful in cancer research to study the effects of mitochondrial dysfunction on cell viability.