Triton X-100 is widely used as a non-ionic surfactant in various laboratory and industrial applications. It is commonly employed to solubilize proteins and lipids in cell lysis buffers, making it essential for protein extraction and purification processes. In immunohistochemistry, it is used to permeabilize cell membranes, allowing antibodies to access intracellular targets. Additionally, Triton X-100 is utilized in cleaning solutions for its ability to break down grease and organic residues. It also finds application in emulsion formulations, such as in paints and coatings, to stabilize mixtures of oil and water. In molecular biology, it is often added to hybridization and washing buffers to reduce non-specific binding. Its versatility and effectiveness make it a key component in many scientific and industrial workflows.