Used primarily as a derivatization agent in gas chromatography (GC) and mass spectrometry (MS) for the analysis of compounds containing active hydrogen atoms, such as alcohols, phenols, and carboxylic acids. It effectively converts these functional groups into their trimethylsilyl (TMS) derivatives, enhancing their volatility and thermal stability, which improves detection and separation. Commonly applied in pharmaceutical, environmental, and biochemical research for accurate identification and quantification of analytes. Its inclusion of trimethylchlorosilane (TMCS) acts as a catalyst, accelerating the derivatization process and ensuring complete reaction.