BSTFA is widely used as a derivatization agent in gas chromatography (GC) and mass spectrometry (MS) to enhance the volatility and stability of compounds. It is particularly effective in converting polar functional groups, such as hydroxyl, carboxyl, and amine groups, into their trimethylsilyl (TMS) derivatives, which are more suitable for analysis. This makes BSTFA a valuable tool in the analysis of complex mixtures, such as environmental samples, pharmaceuticals, and biological fluids. Additionally, BSTFA is used in the preparation of samples for GC-MS in metabolomics and forensic studies, where it helps in the accurate identification and quantification of analytes. Its ability to react quickly and efficiently at relatively low temperatures further contributes to its widespread use in analytical chemistry.