Used primarily as a derivatization agent in gas chromatography (GC) and mass spectrometry (MS) to enhance the volatility and stability of compounds. It is particularly effective for analyzing polar compounds such as amino acids, carbohydrates, and organic acids by converting them into trimethylsilyl (TMS) derivatives. This process improves peak resolution and detection sensitivity in analytical instruments. Additionally, it is employed in the preparation of samples for NMR spectroscopy to simplify complex spectra. Its application extends to the pharmaceutical and biochemical industries for the analysis of metabolites and other small molecules.