This compound, 2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate, is a protected glycosyl isothiocyanate primarily used in the synthesis of thioglycosides and glycosyl thioureas. It acts as a key building block for introducing the acetyl-protected β-D-glucopyranosyl moiety via its isothiocyanate group, which reacts with nucleophiles such as amines to form thiourea linkages and other glycosylated compounds. These derivatives are valuable intermediates in carbohydrate chemistry and are employed in developing bioactive molecules, including glycoconjugates, with applications in drug discovery, biochemical research, enzyme inhibitor studies, and modification of natural products to improve biological activity or stability. Its role in glycosylation reactions positions it as an essential reagent in organic synthesis and medicinal chemistry.