Used primarily in analytical chemistry for the detection and quantification of nickel and palladium. It forms colored complexes with these metals, making it useful in spectrophotometric analysis. Additionally, it serves as a reagent in biochemical research for studying enzyme activities, particularly those involving acetylcholinesterase. In organic synthesis, it acts as a precursor for various heterocyclic compounds. Its ability to react with carbonyl groups also makes it valuable in the determination of urea in blood and urine samples, aiding in medical diagnostics.