Neocuproine hydrochloride monohydrate is widely used as a chelating agent in biochemical research, particularly for the detection and quantification of copper ions. It forms a stable complex with copper(I), which is utilized in colorimetric assays to measure copper levels in various samples, including biological fluids and environmental samples. This compound is also employed in studies involving redox chemistry, where it helps to stabilize copper in its +1 oxidation state, facilitating the investigation of electron transfer processes. Additionally, it serves as a reagent in organic synthesis, particularly in reactions where copper catalysis is involved. Its ability to selectively bind copper makes it valuable in analytical chemistry for developing sensitive and specific detection methods.