This compound is a selective reagent for the spectrophotometric determination of iron(II) ions in analytical chemistry and biochemical research. It forms a stable, red-colored complex with Fe(II) that exhibits strong absorbance at approximately 562 nm, enabling sensitive and quantitative detection in complex matrices such as biological fluids, tissues, environmental samples, and food products. Its chelating properties facilitate precise iron quantification, and it is widely used in studies of iron metabolism, oxidative stress, and related cellular processes. The compound's water solubility and stability under physiological conditions make it ideal for diagnostic and research applications.