Triton X-100 is widely used as a non-ionic surfactant in various laboratory and industrial applications. In biological research, it is commonly employed to solubilize proteins and disrupt cell membranes, making it a key component in cell lysis buffers. It is also used in immunoassays, such as ELISA and Western blotting, to enhance antibody binding and reduce background noise. In the field of microscopy, Triton X-100 is added to staining solutions to improve permeability of cell membranes, allowing dyes and antibodies to penetrate more effectively. Additionally, it is utilized in cleaning products and formulations for its ability to emulsify oils and grease, making it effective in industrial and household cleaners. In pharmaceutical manufacturing, it aids in the stabilization and solubilization of active ingredients in drug formulations.