Thioacetamide is widely used as a source of sulfide ions in analytical chemistry, particularly in qualitative inorganic analysis for the precipitation of metal sulfides. It slowly hydrolyzes in aqueous solutions to release hydrogen sulfide, allowing controlled separation of metal ions in different groups, especially in the analysis of heavy metals like lead, mercury, and copper. It is also employed in the synthesis of sulfur-containing organic compounds and in the preparation of semiconductor nanomaterials, such as metal sulfide nanoparticles and quantum dots, due to its ability to provide a slow and uniform release of sulfur. In industrial applications, thioacetamide is used in the vulcanization of rubber and as a reagent in textile and leather processing. Additionally, it finds use in electroless plating processes and as a stabilizer in certain chemical formulations. In research, it serves as a hepatotoxicant in laboratory models to induce liver injury in experimental animals, helping in the study of liver diseases and potential therapeutic agents.