Widely used as a silylating agent in analytical chemistry, especially in gas chromatography (GC) and mass spectrometry (MS). It efficiently derivatizes polar functional groups such as hydroxyl, carboxyl, and amino groups by replacing active hydrogens with trimethylsilyl groups. This enhances the volatility and thermal stability of analytes, improving their separation and detection. Commonly applied in the analysis of steroids, bile acids, drugs, and metabolites in biological samples. Its high reactivity and clean reaction profile reduce side products, making it preferred for sensitive and accurate quantitative analysis. Also used in forensic toxicology and pharmaceutical quality control due to its reproducible derivatization results.