Used as a reagent in organic synthesis, particularly in the development of colorimetric sensors for detecting electrophilic compounds such as alkylating agents and reactive species. The pyridine nitrogen acts as a nucleophile, allowing selective reactions that produce visible color changes, making it valuable in analytical chemistry for qualitative and quantitative testing. Also employed in research settings to study reaction mechanisms involving nitroaromatic systems, as an intermediate in the preparation of more complex heterocyclic compounds, and in biochemical studies of enzyme behavior and biomolecules interacting with specific functional groups. Its UV-visible absorption properties enable effective use with spectroscopic techniques.