Used primarily as a silylating agent in analytical chemistry, especially in gas chromatography-mass spectrometry (GC-MS). It introduces the tert-butyldimethylsilyl (TBDMS) group to polar functional groups such as hydroxyl, amino, and carboxyl groups in organic molecules. This derivatization increases the volatility and thermal stability of analytes, improving their chromatographic behavior and detection sensitivity. Commonly applied in the analysis of steroids, drugs, metabolites, and other biologically relevant compounds. The reaction is typically carried out under mild conditions and is suitable for complex mixtures.