Widely used as a silylating agent in analytical chemistry, especially in gas chromatography (GC) and mass spectrometry (MS). It efficiently introduces trimethylsilyl groups to replace active hydrogens in compounds containing -OH, -NH, or -COOH functional groups, enhancing their volatility and thermal stability for GC analysis. This derivatization improves peak resolution and detection sensitivity. Commonly applied in the analysis of steroids, bile acids, drugs, and metabolites in biological samples. Also used in organic synthesis to protect functional groups during multi-step reactions. Its high reactivity and ability to work under mild conditions make it a preferred choice in both research and quality control laboratories.