Used as a silylating agent in analytical chemistry, particularly in gas chromatography (GC) and mass spectrometry (MS). It modifies polar functional groups such as hydroxyl, carboxyl, and amino groups in organic compounds, making them more volatile and thermally stable for GC analysis. The mixture efficiently derivatizes steroids, fatty acids, phenols, and other bioactive molecules, improving peak shape and detection sensitivity. Commonly applied in metabolite profiling, environmental testing, and pharmaceutical analysis where accurate identification and quantification of complex mixtures are required. The reaction is typically fast and performed under mild conditions, often at room temperature, making it suitable for high-throughput sample preparation.