Magnesium ionophore I is primarily used in the development of ion-selective electrodes (ISEs) for the detection and measurement of magnesium ions (Mg²⁺) in various samples. Its application is crucial in analytical chemistry, particularly in environmental monitoring, clinical diagnostics, and industrial processes where accurate magnesium quantification is essential. In clinical settings, it aids in assessing magnesium levels in biological fluids like blood and urine, which is vital for diagnosing and managing conditions such as hypomagnesemia or hypermagnesemia. In environmental science, it helps monitor magnesium concentrations in water bodies, ensuring compliance with safety standards. Additionally, it is employed in research to study magnesium's role in biological systems and its interactions with other ions. The ionophore's selectivity and sensitivity make it a valuable tool for precise magnesium analysis across diverse fields.