Sodium 1-heptanesulfonate monohydrate is widely used as an ion-pairing agent in high-performance liquid chromatography (HPLC). It helps improve the separation of polar compounds by forming ion pairs, enhancing retention and resolution. This chemical is also employed in the analysis of pharmaceuticals, enabling the detection and quantification of active ingredients. Additionally, it serves as a surfactant in various industrial applications, aiding in the stabilization of emulsions and the solubilization of hydrophobic substances. Its role in biochemical research includes facilitating protein purification and analysis by modifying surface interactions.