Used as a redox indicator in analytical chemistry, particularly in the determination of ascorbic acid (vitamin C) content in various samples. It is employed in titrimetric methods where it changes color based on the oxidation state, allowing for precise endpoint detection. Additionally, it serves as an electron acceptor in biochemical assays to study enzymatic activities, especially in photosynthesis research. Its application extends to microbiology for assessing the metabolic activity of certain microorganisms. The compound is also utilized in educational laboratories to demonstrate redox reactions and principles of titration.