Sodium ionophore III is widely used in the development of ion-selective electrodes (ISEs) for detecting sodium ions in various samples. Its primary application is in clinical diagnostics, where it helps measure sodium levels in blood and other biological fluids, aiding in the assessment of electrolyte balance and kidney function. It is also employed in environmental monitoring to analyze sodium concentrations in water sources, ensuring water quality and safety. Additionally, it finds use in research laboratories for studying ion transport mechanisms and developing sensors for sodium detection in complex matrices. Its selectivity and sensitivity make it a valuable tool in analytical chemistry and biomedical applications.