Benzoylcholine Iodide is primarily used in biochemical research as a substrate for studying the activity of cholinesterase enzymes. These enzymes are critical in breaking down acetylcholine, a neurotransmitter, in the nervous system. By measuring the hydrolysis of Benzoylcholine Iodide, researchers can assess the efficiency and inhibition of cholinesterases, which is valuable in understanding neurological disorders and developing therapeutic agents. Additionally, it serves as a tool in pharmacological studies to evaluate the effects of drugs targeting the cholinergic system. Its application is essential in advancing knowledge related to neurodegenerative diseases and the development of treatments for conditions like Alzheimer’s disease.