Acetylcholine bromide is primarily used in scientific research to study the function of the cholinergic system, which plays a critical role in the nervous system. It is often applied in experiments to investigate the effects of acetylcholine on various tissues, particularly in the heart and smooth muscles, due to its ability to mimic the action of the neurotransmitter acetylcholine. This compound is also utilized in pharmacological studies to understand receptor interactions and to develop drugs targeting conditions like myasthenia gravis, glaucoma, and Alzheimer's disease. Additionally, it serves as a tool in electrophysiology to study synaptic transmission and neuronal communication. Its application is essential in advancing knowledge of neurological and muscular disorders.