AF38469 is primarily utilized in research settings as a potent and selective inhibitor of acetylcholinesterase (AChE). Its application is significant in studying the role of AChE in neurological processes, particularly in understanding conditions like Alzheimer's disease and other cognitive disorders. By inhibiting AChE, it helps researchers explore the mechanisms of acetylcholine regulation in the brain, providing insights into potential therapeutic strategies. Additionally, AF38469 is used in pharmacological studies to assess the effects of AChE inhibition on memory, learning, and synaptic plasticity, contributing to the development of novel treatments for neurodegenerative diseases.