Used primarily as a choline esterase inhibitor in biochemical research, this compound serves as a mimic of acetylcholine with enhanced stability. It is employed in studies involving neurotransmission, particularly to probe the function of cholinergic receptors and enzymes in neural and muscular tissues. Due to its fluorinated alkyl group, it exhibits increased metabolic resistance, making it valuable in assays requiring prolonged activity. It is also utilized in the development and testing of antidotes for neurotoxic agents, including organophosphate poisoning. Its quaternary ammonium structure allows for good solubility in aqueous systems, facilitating use in physiological buffers and cell-based experiments.