Neostenine is primarily used in research settings for its biological activity, particularly in studies related to enzyme inhibition and neuropharmacology. It has shown potential in modulating cholinesterase activity, making it a compound of interest in the development of treatments for neurodegenerative conditions such as Alzheimer's disease. Its structure allows interaction with key neural receptors, supporting investigations into cognitive enhancement and memory regulation. Additionally, Neostenine serves as a chemical scaffold in the synthesis of derivatives aimed at improving selectivity and potency in central nervous system agents. Due to its natural origin and specific activity, it is also used as a reference standard in alkaloid profiling and plant-based drug discovery.