Eseroline is primarily used in scientific research for its pharmacological effects on the central nervous system. It acts as a stimulant and has been studied for its ability to cross the blood-brain barrier, making it useful in neuropharmacology studies. Researchers utilize eseroline to investigate cholinergic pathways due to its relationship with acetylcholinesterase inhibition, although it is less potent than related compounds like physostigmine. It also serves as a metabolic byproduct and intermediate in the breakdown of certain alkaloids, aiding in the study of drug metabolism. Due to its psychoactive properties, it has been referenced in studies on hallucinogens and CNS stimulants, though it is not used clinically in medicine.