L-690330 is primarily utilized in research settings for its role as a potent and selective inhibitor of the enzyme acetylcholinesterase. This property makes it valuable in studies aimed at understanding the biochemical pathways involving acetylcholine, a critical neurotransmitter in the nervous system. Researchers employ L-690330 to investigate the effects of acetylcholinesterase inhibition on cognitive functions, memory processes, and neuromuscular transmission. Additionally, it serves as a tool compound in the development of therapeutic agents for neurodegenerative disorders such as Alzheimer's disease, where acetylcholinesterase activity is a key target. Its application extends to exploring potential treatments for myasthenia gravis and other conditions linked to cholinergic system dysfunction.