Used as a chiral resolving agent in enantiomeric separations, particularly in capillary electrophoresis and HPLC. Its structure allows strong interactions with enantiomers of acidic and neutral compounds through π-π stacking, electrostatic forces, and hydrophobic effects. Commonly applied in pharmaceutical analysis for separating racemic mixtures of drugs containing carboxylic acid or phenolic groups. Also employed in studies involving chiral recognition mechanisms due to its rigid aromatic framework and permanent positive charge, enhancing selectivity and detection sensitivity.