(2R,3R)-2-Amino-3-(tert-butoxy)butanoic acid is primarily used in the field of organic synthesis and pharmaceutical research. It serves as a key intermediate in the preparation of complex molecules, particularly in the synthesis of chiral compounds. Its stereochemistry makes it valuable for constructing peptides and peptidomimetics, which are essential in drug discovery and development. Additionally, it is utilized in the study of enzyme inhibitors and receptor ligands, aiding in the design of biologically active compounds. The tert-butoxy group provides steric protection, making it useful in selective chemical reactions.