(1S,2S)-2-((tert-Butoxycarbonyl)amino)cyclopentanecarboxylic acid is primarily used in the synthesis of peptides and other biologically active compounds. It serves as a key intermediate in the production of chiral molecules, particularly in the pharmaceutical industry, where it is utilized to create enantiomerically pure drugs. The compound is often employed in the preparation of cyclopentane-based scaffolds, which are important in drug discovery for their structural rigidity and ability to mimic peptide bonds. Additionally, it is used in the development of protease inhibitors and other therapeutic agents targeting specific enzymes or receptors. Its tert-butoxycarbonyl (Boc) protecting group makes it valuable in solid-phase peptide synthesis, allowing for selective deprotection and further functionalization.