(1R,4S)-4-((tert-Butoxycarbonyl)amino)cyclopent-2-enecarboxylic acid is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the preparation of more complex molecules, particularly in the development of drugs and bioactive compounds. The tert-butoxycarbonyl (Boc) protecting group in the molecule is crucial for safeguarding the amino functionality during multi-step synthetic processes, allowing for selective reactions to occur elsewhere in the molecule. This compound is often employed in the synthesis of peptidomimetics and other small molecules that mimic peptide structures, which are valuable in drug discovery for targeting various diseases. Additionally, its cyclopentene ring structure makes it a useful building block for creating constrained analogs of biologically active compounds, enhancing their stability and potency.