Used as a chiral derivatizing agent in analytical chemistry, particularly for the separation and determination of enantiomers in complex mixtures. Its structure allows selective reaction with amino groups in chiral amines or amino acids, forming diastereomeric derivatives that can be effectively resolved using techniques like HPLC or GC. The presence of the fluorine atom and nitro groups enhances detectability through UV or mass spectrometry, making it valuable in pharmaceutical analysis where precise stereochemical identification is critical. Also employed in research settings to study reaction mechanisms involving chiral intermediates.