This chiral compound, (R)-(-)-N-(3,5-Dinitrobenzoyl)-α-phenylethylamine, is widely used as a reference standard in enantiomeric separation and chiral analysis. It is typically prepared by acylation of (R)-α-phenylethylamine with 3,5-dinitrobenzoyl chloride and serves as a model analyte for evaluating the performance of chiral stationary phases in chromatographic techniques, such as HPLC. The electron-withdrawing dinitrobenzoyl group provides a strong chromophore for sensitive UV detection, while the chiral center allows for clear differentiation of enantiomers. It is essential in analytical chemistry for method development, validation, and determination of enantiomeric purity, and in the pharmaceutical industry for quality control during the synthesis and analysis of chiral drugs.