Used as a highly selective fluorescent derivatization reagent for chiral amines and amino acids in analytical chemistry. Its primary application lies in high-performance liquid chromatography (HPLC) and capillary electrophoresis, where it enables sensitive detection of enantiomers due to its strong fluorescence properties. The reagent reacts selectively with primary amines, forming stable amide bonds that allow for clear differentiation and quantification of R- and S-enantiomers. Due to the presence of the chloroformyl group and sulfonyl moiety, it enhances electrophilic reactivity and detection sensitivity under UV or fluorescence detectors. Commonly employed in pharmaceutical analysis, biofluid monitoring, and metabolic studies where precise chiral discrimination and low detection limits are critical.